QCM Theory

The Principles of QCM-I (QCM-D) - Figure 1

The Principles of QCM-I

Quartz Crystal Microbalance with Impedance Analysis (QCM-I) and Dissipation Monitoring (QCM-D) The Quartz Crystal Microbalance is a well-established and sensitive technique used to measure the interactions of molecules, polymers and biological assemblies with a sensor surface, in air or liquid, label-free and in real time. It is based on the change in resonant frequency of […]

The Density and Refractive Index of Adsorbing Protein Layers

Abstract The structure of the adsorbing layers of native and denatured proteins (fibrinogen, γ-immunoglobulin, albumin, and lysozyme) was studied on hydrophilic TiO2 and hydrophobic Teflon-AF surfaces using the quartz crystal microbalance with dissipation and optical waveguide lightmode spectroscopy techniques. The density and the refractive index of the adsorbing protein layers could be determined from the […]

Formation of supported bacterial lipid membrane mimics

Abstract In recent years, a large effort has been spent on advancing the understanding of how surface-supported membranes are formed through vesicle fusion. The aim is to find simple model systems for investigating biophysical and biochemical interactions between constituents of cell membranes and, for example, drugs and toxins altering membrane function. Designing and controlling the […]

Elongation kinetics of polyglutamine peptide fibrils: a quartz crystal microbalance with dissipation study.

Abstract Abnormally expanded polyglutamine domains in proteins are associated with several neurodegenerative diseases, including Huntington’s disease. Expansion of the polyglutamine (polyQ) domain facilitates aggregation of the affected protein, and several studies directly link aggregation to neurotoxicity. Studies of synthetic polyQ peptides have contributed substantially to our understanding of the mechanism of aggregation. In this report, […]

Attachment of Pathogenic Prion Protein to Model Oxide Surfaces

Abstract Prions are the infectious agents in the class of fatal neurodegenerative diseases known as transmissible spongiform encephalopathies, which affect humans, deer, sheep, and cattle. Prion diseases of deer and sheep can be transmitted via environmental routes, and soil is has been implicated in the transmission of these diseases. Interaction with soil particles is expected […]

Bioaffinity Sensor Based on Nanoarchitectonic Films

Bioaffinity Sensor Based on Nanoarchitectonic Films: Control of the Specific Adsorption of Proteins through the Dual Role of an Ethylene Oxide Spacer Abstract The identification and quantification of biomarkers or proteins is a real challenge in allowing the early detection of diseases. The functionalization of the biosensor surface has to be properly designed to prevent […]