The electrostatically driven adsorption of polymer and nano-particle multilayers is a well-established method for the physical and chemical transformation of surfaces to produce highly tailored and often “smart”, environmentally sensitive interfaces. The deposition process can be sensitively monitored using QCM-I, which provides information on the hydrated mass of the multilayer as well as its physical […]
Pump Flow Control with Manual Sample Introduction It is recommended that samples are introduced to the QCM-I flow-cells using a pump to control the flow. In the simplest setup, shown in figure 1, a peristaltic pump* is used to suck buffer directly from a beaker or other container. Sample is introduced to the flow-cell by […]
MicroVacuum’s high performance QCM-I device is a Quartz Crystal Microbalance System with impedance analyses and dissipation monitoring (QCM -D), the Glove-Box QCM-I takes the high performance QCM-I instrument into a format suitable for mounting in a Glove-box or controlled atmosphere chamber and also includes the option of a wider temperature range e.g. between -30 °C and 80 °C.
Quartz Crystal Microbalance with Impedance Analysis (QCM-I) and Dissipation Monitoring (QCM-D) The Quartz Crystal Microbalance is a well-established and sensitive technique used to measure the interactions of molecules, polymers and biological assemblies with a sensor surface, in air or liquid, label-free and in real time. It is based on the change in resonant frequency of […]
We are very proud that both of our OWLS and QCM-I biosensor systems were used in the recent comparative study published on Nature.com See the abstract below and visit Nature.com to read the full publication. In situ viscoelastic properties and chain conformations of heavily hydrated carboxymethyl dextran layers: a comparative study using OWLS and QCM-I […]
Variations in Coupled Water, Viscoelastic Properties, and Film Thickness of a Mefp-1 Protein Film during Adsorption and Cross-Linking: A Quartz Crystal Microbalance with Dissipation Monitoring, Ellipsometry, and Surface Plasmon Resonance Study
Abstract We have measured the time-resolved adsorption kinetics of the mussel adhesive protein (Mefp-1) on a nonpolar, methyl-terminated (thiolated) gold surface, using three independent techniques: quartz crystal microbalance with dissipation monitoring (QCM-D), surface plasmon resonance, and ellipsometry. The QCM-D and ellipsometry data shows that, after adsorption to saturation of Mefp-1, cross-linking of the protein layer […]
Simultaneous determination of optical and acoustic thicknesses of protein layers using surface plasmon resonance spectroscopy and quartz crystal microweighing
Abstract The optical and the acoustic thicknesses of protein layers during adsorption were simultaneously determined by a combination of surface plasmon resonance spectroscopy and quartz crystal microweighing. Coupling of the surface plasmon was achieved by etching a diffraction grating into the quartz surface prior to the deposition of the metal electrode. The evolution of the […]
Abstract The structure of the adsorbing layers of native and denatured proteins (fibrinogen, γ-immunoglobulin, albumin, and lysozyme) was studied on hydrophilic TiO2 and hydrophobic Teflon-AF surfaces using the quartz crystal microbalance with dissipation and optical waveguide lightmode spectroscopy techniques. The density and the refractive index of the adsorbing protein layers could be determined from the […]
Abstract In recent years, a large effort has been spent on advancing the understanding of how surface-supported membranes are formed through vesicle fusion. The aim is to find simple model systems for investigating biophysical and biochemical interactions between constituents of cell membranes and, for example, drugs and toxins altering membrane function. Designing and controlling the […]
Elongation kinetics of polyglutamine peptide fibrils: a quartz crystal microbalance with dissipation study.
Abstract Abnormally expanded polyglutamine domains in proteins are associated with several neurodegenerative diseases, including Huntington’s disease. Expansion of the polyglutamine (polyQ) domain facilitates aggregation of the affected protein, and several studies directly link aggregation to neurotoxicity. Studies of synthetic polyQ peptides have contributed substantially to our understanding of the mechanism of aggregation. In this report, […]